The basic structure of a biological microscope
1. Mirror holder. Located at the bottom of the microscope, used to support the entire scope.
2. Mirror arm. Located behind the lens barrel, usually in the shape of an arch, it is used to support the lens barrel and grip the microscope when moving it.
3. Tube. Located above the microscope, connected to the eyepiece above and the objective converter below.
4. Objective lens converter. The turntable located below the lens barrel usually has 3-4 circular holes, which can be equipped with objective lenses of different magnifications, allowing each objective lens to form an amplification system through the lens barrel and eyepiece.
5. Mobile station. Also known as a stage, workbench, or mirror stage, used for placing specimens. There are two metal compression clamps called specimen clamps on the mobile platform, which are used to fix glass slide specimens. Some stages are equipped with slide pushers to move specimens, while others can move themselves.
6. Focusing device. In order to obtain clear images, it is necessary to adjust the distance between the objective lens and the specimen, so that the focus of the objective lens is aligned with the specimen. This operation is called focusing. Focusing is achieved through the coarse focus knob and fine focus knob.
7. Objective lens. The objective lens converter installed below the lower end of the lens barrel is also known as the lens adapter because it is close to the object being viewed. The objective lens is the most important component that determines the performance of a microscope, such as resolution. The function of the objective lens is to magnify the specimen into an inverted image for the first time. A microscope is equipped with several objective lenses, each consisting of several lenses with different spherical radii. The smaller the aperture of the lens at the lower end of the objective lens and the longer the barrel, the higher its magnification; Otherwise, the opposite is true. There are low magnification and high magnification objective lenses, and their magnification is usually engraved on the lens barrel of the objective lens, such as 4x, 8x, 10x,..., 100x, representing 4x, 8x, 10x..., 100x respectively. Among them, 40-65 times is called high-power objective lens, and 90 or 100 times is called oil immersed objective lens. Olympus objectives can provide microscope objectives that can meet the vast majority of digital imaging needs, and have launched specialized objectives for various applications such as tissue culture observation, laboratory work, cell culture monitoring, multiphoton imaging, and confocal/super-resolution microscopy. [3-4]
8. Eyepiece. Installed at the upper end of the lens barrel, also known as the eyepiece, because it is close to the observer's eyes. The function of an eyepiece is to further enlarge the real image magnified by the objective lens into a standing virtual image, which is equivalent to a magnifying glass. But it does not increase the resolution of the microscope. There is a black line in the field of view of the eyepiece called the "pointer", which can indicate the observed area. As needed, a micrometer can also be installed inside the eyepiece to measure the size of the observed object. Generally, microscopes are equipped with several eyepieces with different magnifications, which are engraved on the eyepiece frame, such as 5 ×, 10 ×, 15 ×, etc. (total magnification of the microscope=objective magnification x eyepiece magnification).
9. Spotlight. Installed on the bracket below the mobile platform, it mainly consists of a condenser lens and an aperture stop. The function of a spotlight is to gather the light from the mirror and increase the illumination of the specimen. Aperture stop, also known as aperture, is used to adjust the intensity of light. Under the aperture stop, there is usually a circular filter holder that can be used to place the filter according to the needs of mirror inspection.
10. Light source. Usually installed inside the microscope mount to provide a light source.